Infrastructure

 

The infrastructure of the research center has been intensively developed in the last years, through the financial support of several national and international research projects of the research group members. Furthermore several instruments were obtained by donations from companies from Romania, our long-time collaborators (Terapia Ranbaxy, Farmec, Kynetix).

The infrastructure is organized over several laboratories:

  1. Synthesis lab (lab 11 și lab 16)
  2. Fermentation lab (lab 36)
  3. Proteomics and genomics lab (lab 32)
  4. Biotransformation and bioanalytic lab I, II (lab 33-34)
  5. LC-MS analysis platform (lab 34)
  6. Microreactor lab (lab 54)

Furthermore the research center have access to the strategic research facilities of the Faculty of Chemistry and Chemical Engineering of Babes-Bolyai University (NMR spectrometers. Atomic absorption-emission spectrometer, etc.).

Regulation for the use of biotransformation and biocatalysis research center equipment.

 

Equipment list

 
 

Prometheus NT.48 (Manufacturer: NanoTemper Technologies GmbH 2016)

 

Differential Scanning Fluorimeter which allows the high-throughput determination of protein stabilities, based on the detection of the intrinsec fluorescence of Trp and Tyr residues of proteins (sample concentration range: 5 µg/ml - 250 mg/ml).

Technical description
 
 

Transmission electron microscope TEM Hitachi H-7650 (Manufacturer: Hitachi Group 2010)

 

Registration of high resolution and contrast images, necessary for analytical, biomedical, materials sciences.

Technical description
 
 

Protein purification system FPLC-Äkta (Manufacturer: GE Health Care 2007)

 

Pump with two channels, detector UV 900, conductometer, fraction collector Frac-950, Unicorn software.

Technical description
 
 

Microplate reader - TECAN 10M Spark (Manufacturer: Tecan Group Ltd.)

 

Technical description
 
 

Lyophilizer - ALPHA 1-2 LDPlus (Manufacturer: Martin Christ 2007)

equipped with high-vacuum (< 0.040 atm) pump

Technical description
 
 
 

Agilent LC-MS system (Manufacturer: Agilent Technologies Inc. 2006)

 
 

Analysis and characterization of compounds, inclusive from a complex mixture (biological samples). Equipped with specific software and databases as well as the necessary auxiliar instrumentations.

Technical description
 
 

Chemidoc imaging system (Manufacturer: BioRad)

 
 

Technical description
 
 

Flow chemistry system - SpinSplit (Manufacturer: SpinSplit Technical Research and Development LLC)

 
 

Technical description
 
 

Ultrasonic Microwave Chemical Reactor UMCR-SM50 (Manufacturer: Col-Int Tech (USA), LLC)

 
 

Technical description
 
 

HPLC Agilent 1200 (Manufacturer: Agilent Technologies Inc. 2006)

 

Quaternary pump, degasor, DAAD detector, Chemstation software - equipped with diverse standard and chiral columns.

 
 

HPLC Agilent 1260 (Manufacturer: Agilent Technologies Inc. 2012)

 

Quaternary pump, degasor, DAAD detector, ELSD detector (with N2 generator), Chemstation software - equipped with diverse standard and chiral columns.

 
 

Gas chromatograph GC Agilent MF 235330 (Manufacturer: Agilent Technologies Inc. 2009)

FID detector, autosampler, Chemstation software –equipped with diverse standard and chiral columns.

 
 

UV-VIS Agilent 8453 spectrophotometer (Manufacturer: Agilent Technologies Inc. 2010)

 

Analysis in UV-VIS domain, equipped with thermostat, multi-channel sample system with 8 positions and specific software including enzyme kinetics and other applications.

 
 

UV-VIS spectrophotometer Selecta 3100
Manufacturer: Selecta 2016

Analysis in UV-VIS domain, specific software including enzyme kinetics and other applications.

 
 

Bioreactor MS-F1 (Manufacturer: Bionet Engineering Co 2010)

 

Autoclavable 4L bioreactor, equipped with pums, filters, specific sensors / electrodes and software for control.

 
 

Termocycler Eppendorf Mastercycler Pro (Manufacturer: Eppendorf Co. 2007)

 

Thermocycler with 24 positions, useful for PCR amplification procedures of DNA samples.

 
 

Microstar 17R microcentrifuge (Manufacturer: VWR Co. 2015)

Max. 13000 rpm, 24 position, with cooling system..

 
 

Horizontal and vertical electrophoresis systems with gel-casting system.
Manufacturer: Biorad 2015, 2016

Preparation and migration of 1-4 gels used for the analysis of proteins and nucleic acids.

 
 

Vertical shaker Biosan Multi Bio RS-24
Manufacturer: Biosan 2015

5-30 rpm, vertical rotations of 360°

 
 

Transiluminator- UVP Bioimaging
Manufacturer: UVP Analytic Jena 2015

Filters for λ = 300, 302, 312 nm useful for detection of DNA in gels with dimensions of 15x15 to 25x26 cm.

 
 

Titramax 1000 shaker
Manufacturer: Heidolph 2014, 2016

32-48 sample positions, 150-1350 rpm, adjustable time range: 1-120 min, temperature range 20 - 65°

 
 

Vibramax 100 shaker
Manufacturer: Heidolph 2009, 2015

150-1350 rpm, adjustable time range: 1-120 min

 
 

Incubator Multi Stack Shaking Labtech
Manufacturer: Daihan Labtech India Pvt. Ltd. 2007

100-300 rpm, 25-70°C, 10-2000 mL glassware

 
 

Incubator Innova 40 R (Manufacturer: New Brunswick 2016)

25-500 rpm, temperature range 25-70 °C, 10-2000 mL

 
 

Raypa AES-40 autoclave (Manufacturer: Raypa Lab. Equipment 2007)

40 L, with various predefined sterilisation programs

 
 

Presoclav 80 autoclave (Manufacturer: Selecta 2016)

40 L, with various predefined sterilisation programs

 
 

Titrator 902 Titrando – pH STAT
Manufacturer: Methrom 2012

pH measurements, automated titration, pH monitoring and automated adjustment, 3 point calibration, etc.

 
 

ADP220 Digital polarimeter (Manufacturer: Bellingham Stanley 2005)

with cuvettes of 1,2 and 10 ml.

 
 

GFL Water Still (Manufacturer: Jena 2015)

system for double-distilled water with a production capacity of 2 l/h

 
 

Deep-freezer Premium U410 Ultra-Low Temperature (Manufacturer: New Brunswick 2014)

-81 °C, volume of 80 l.