Publications

Project scientific papers published in ISI journals


1. Dorina Casoni, Costel Sarbu, Comprehensive evaluation of lipophilicity of biogenic amines and related compounds using different chemically bonded phases and various descriptors, Journal of Separation Science, 35 (2012) 915-921. (FI = 2,591) Abstract
ABSTRACT


2. Rodica Domnica Nascu-Briciu, Costel Sarbu, A comparative study concerning the chromatographic behaviour and lipophilicity of certain natural toxins, Journal of Separation Science, 35 (2012) 1059-1067. (FI = 2,591) Abstract
ABSTRACT


3. Costel Sarbu, Rodica Domnica Nascu-Briciu, Dorina Casoni, Agata Kot-Wasik, Andrzej Wasik, Jacek Namiesnik, Chromatographic lipophilicity determination using large volume injections of the solvents non-miscible with the mobile phase, Journal of Chromatography A, 1266 (2012) 53– 60. (FI = 4,612) Abstract
ABSTRACT


4. Ioana Tuhutiu, Dorina Casoni, Costel Sarbu, High sensitive and selective HPTLC method assisted by digital image processing for simultaneous determination of catecholamines and related drugs, Talanta, 114 (2013), 117-123. (FI = 3,498) Abstract
ABSTRACT


5. Rodica Domnica Nascu-Briciu, Costel Sarbu, Lipophilicity of oils and fats estimated by TLC, Journal of Separation Science, 36 (2013) 1317-1326. (FI = 2,591) Abstract
ABSTRACT


6. Dorina Casoni, Costel Sarbu, Comprehensive evaluation of biogenic amines and related drugs antiradical activity using reactive 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, Central European Journal of Chemistry, 11 (2013) 679-688. (FI = 1,167) Abstract
ABSTRACT


7. Ioana Anamaria Sima, Dorina Casoni, Costel Sarbu, Simultaneous determination of carbidopa and levodopa using a new tlc method and a free radical as detection reagent, Journal of Liquid Chromatography & Related Technologies, 36 (2013) 2395-2404. (FI = 0,565) Abstract
ABSTRACT


8. Tuhutiu Ioana, Rodica Domnica Nascu-Briciu, Costel Sarbu, Simultaneous Determination of Levodopa and Carbidopa in Pharmaceuticals by Principal Component Regression, Revue Roumaine de Chimie 58 (2013) 705-710 Abstract
ABSTRACT


9. Ioana Fort, Ileana E. Silai, Dorina Casoni, Graziella L. Turdean, Electrochemical study of isoprenaline and epinephrine at platinum-nanoparticles-chitosan modified graphite electrode. Studia UBB Chemia, LVIII 4 (2013) 193-202 Abstract
Two catecholamines (epinephrine and isoprenaline) were studied by cyclic (CV) and square-wave voltammetry (SWV) at bare graphite (G) and Pt nanoparticles (Pt-NP) - chitosan modified graphite electrodes (G/Pt-NPChitosan). The obtained results shown similarly redox behavior for both investigated compounds by mentioned electrochemical techniques at the investigated electrodes, consisting in two pairs of peaks (peak I and II) corresponding to a quasi-reversible processes. The difference between the structures of compounds doesn’t influence the electrochemical parameters values. As expected, the presence of the Pt nanoparticles acts as a diffusion barrier on the electrode surface leading to a decrease of the current intensities values, when electrodes were investigated by CV and SWV. The G/Pt-NPChitosan nanocomposites matrix can be satisfactorily used for detecting catecholamines.


10. Ileana E. Silai, Graziella L. Turdean, Dorina Casoni, Electrochemical behavior of some catecholamines investigated by cyclic and square-wave voltammetry. Studia UBB Chemia LVIII 4 (2013) 203-211 Abstract
Electrochemical measurements of the oxidation of organic compounds can be helpful to understand how these compounds are metabolized by living organisms. Cyclic and square-wave voltammetry were used in view to estimate the electrochemical parameters of redox processes involving catecholamines compounds (isoprenaline, epinephrine, levonordefrin, norepinephrine, dopamine, d-DOPA and carbidopa). Redox processes related to catecholamines are quasi-reversible diffusion controlled reactions at a graphite electrode. According to the decreasing of the oxidation potential, the capacity to act as antioxidant of different catecholamines increases in the order: norepinephrine < levonordefrin < epinephrine < isoprenaline or carbidopa < d-DOPA < dopamine. Both series are influenced by the position of –OH and – NH2 in para-alkyl substituents.


11. Dorina Casoni, Costel Sarbu, Comprehensive evaluation of antioxidant activity: A chemometric approach using principal component analysis, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 118 (2014), 343-348. (FI = 1,977) Abstract
ABSTRACT


12. Dorina Casoni, Ioana A. Sima, Costel Sarbu, Thin-layer chromatography an imageprocessing method for the determination of acidic catecholamine metabolites, Journal of Separation Science 37 (2014) 2675-2681 Abstract
A sensitive and convenient method for acidic catecholamine metabolites (including homovanillic acid, vanillylmandelic acid, 3,4-dihydroxymandelic acid, and 3,4- dihydroxyphenylacetic acid) determination was developed based on thin-layer chromatography and image-processing analysis. The metabolites were separated without a prederivatization step using reversed phase RP-18W high-performance plates. The mobile phase composition, detection, and quantification conditions were systematically investigated through several trials. The reaction with 2,2-diphenyl-1-picrylhydrazyl radical allowed specific detection of acidic catecholamine metabolites with a high sensitivity and a wide linear range. The limit of detection and the limit of quantification were in the range of 13–103 and 18–120 ng/spot, respectively, in all cases. Mean recoveries determined were in the range 95–106% for all of the investigated compounds. The proposed method allowed rapid simultaneous determination of acidic catecholamine metabolites from spiked human urine sample.


13. Cobzac Simona, Dorina Casoni, Costel Sarbu, Lipophilicity of amine neurotransmitter precursors, metabolites and related drugs estimated on various TLC plates, Journal of Chromatographic Science 52 (2014) 1095-1103 Abstract
ABSTRACT


14. Ioana Anamaria Sima, Dorina Casoni, Costel Sarbu, Rapid screening method for the diagnosis of adrenal tumors, Revue Roumaine de Chimie, sent for publication, 2014, 59(11-12), 965-970 Abstract
The increased excretion of normetanephrine, metanephrine and 3-methoxytyramine in urine is indicative of the presence of pheochromocytoma, neuroblastoma or paragangliomas, therefore the development of new methods has appeared to be of great importance in the early screening of these diseases. Consequently, measurement of these catecholamine metabolites in spiked urine specimens, has been carried out by applying a rapid and convenient thin-layer chromatography method with DPPH• radical detection and specialized software for quantitative TLC analysis. The obtained results showed recovery rates between 97.96% and 103.48% for all of the investigated compounds, thus making this method suitable for routine screening of adrenal tumors associated with over-production of catecholamines and hyper-excretion of their metabolites.


15. Rodica Domnica Nascu-Briciu, Ioana Anamaria Sima, Costel Sarbu, The lipophilicity of some drugs with tricyclic structure estimated by thin-layer chromatography and computed by various methods Revue Roumaine de Chimie, 2014, 59(11-12), 971-979 Abstract
The lipophilicity of some drugs with tricyclic structure (antidepressants and phenothiazines) was investigated by thin-layer chromatography, on three stationary phases of RP-18, RP-8, and CN. The mobile phases were based on ethanol and different pH buffers (2, 4, 6, 7, 8, 10 and 12). The investigated lipophilicity indices were both experimentally (mRM, RM0, b, f0, PC1/RM) and theoretically expressed (Log D and Log P). The effect of pH modification over the chromatographic behaviour has been evaluated. In order to find an objective manner of quantitative comparison of different chemically bonded stationary phases and evaluate the reliability of the obtained results some comparison procedures based on correlation diagrams and matrices were performed. The results indicated that all the stationary phases were useful for the lipophilicity investigation and that the chromatographic behaviour is highly dependent by the ionization state of the investigated compounds. This is also confirmed through the significant correlations obtained between pKa values and experimental lipophilicity indices.


16. Ioana Anamaria Sima, Mircea Vasile Diudea, Costel Sarbu, Prediction of lipophilicity of catecholamine related compounds based on the hypermolecule concept, Revue Roumaine de Chimie, 2015, 60(7-8), 665-676 Abstract
A set of 38 catecholamine related compounds was submitted to a novel QSAR approach based on weighting and alignment of the molecules over a hypermolecule. Any QSAR approach assumes that a macroscopic property of a chemical compound depends on the molecular structure or the topological indices, which are derived from the molecular topology or geometry. In this study the indices were calculated using TOPOCluj software and these were adjacency, connectivity, detour, distance, IE[CjMax], IE[CjMin], IP[CjMax], IP[CjMin] and Randic. Further, the calculated indices were correlated with the lipophilicity coefficient, logP, using multivariate regression and genetic algorithms.


17. Alexandra Toma, Denisa Leonte, Valentin Zaharia, Costel Sarbu, Dorina Casoni, Heterocycles 37. Lipophilicity of new polyheterocyclic Schiff bases and Mannich bases estimated by thin-layer chromatography and computational methods, Revue Roumaine de Chimie, 2015, 60(7-8), 677-687. Abstract
Lipophilicity of novel potentially anti-inflammatory 1,2,4-triazole derivatives (Schiff and Mannich bases) has been estimated by thin-layer chromatographic method (TLC). The chromatographic retention was measured using phosphate buffer as aqueous component and methanol respectively methanol containing 10% of different upper alcohol (1-butanol and 1-octanol) as the hydrophobic additive of the mobile phase. Different experimental lipophilicity indices (denoted by mRM, RM0 and PC1/RM) were estimated using retention parameters in all cases. Various partition coefficients (log P) values were calculated by means of different software and further correlated with the experimental indices determined using the organic–aqueous eluent system and systems modified with hydrophobic alcohol addition. It was found that values measured in eluent system modified with 1-butanol better correlate with computed lipophilicity parameters. Furthermore, by applying the principal component analysis (PCA) on the experimental values, the similarity and differences of compounds from the lipophilicity point of view were highlighted.


18. Costel Sarbu, Rodica Domnica Nascu-Briciu, Modelling and prediction of lipophilicity for natural compounds with strong biological activity, Studia Universitatis Babes-Bolyai Chemia, 2015, 60(LX), 265-280. Abstract
The goal of this study was to develop high statistical significant models for lipophilicity estimation for a group of 60 compounds with increased toxicity, belonging to alkaloids and mycotoxins. The multiple linear regression modelling was made by means of genetic algorithms as a function of 972 molecular descriptors, computed by ChemOffice and Dragon Plus software and completed by internet available module for Log P computation, ALOGPS 2.1. The compounds classification has been realized using principal component analysis and hierarchical cluster analysis. Data evaluation has been realized by various correlation matrices and relevant graphs. The modelling was made on the basis of 26 compounds with known log Pexp values and the results were validated by means of additional models developed for a series of 20 compounds. The other 6 compounds, which were excluded from the modelling process, were used afterwards as test set for prediction and comparison. The most descriptive models were those retaining four descriptors, and in all models were selected at least one computationally expressed log P value (miLogP, KOWWIN and ALOGP most often). All the obtained results are highly suggestive and offer a very pertinent idea regarding the lipophilicity range of natural compounds of increased toxicity. The models were validated considering various statistical parameters and different correlation matrices defined by high statistical significance.


19. Ioana A. Sima, Costel Sarbu, Rodica D. Na?cu-Briciu, Use of TLC and UV-visible Spectrometry for fingerprinting of dietary supplements, Chromatographia, 2015, 78, 929-935. Abstract
We have studied dietary supplements containing sea-buckthorn (Hippophae rhamnoides), bilberry (Vaccinium myrtillus), and cranberry (Vaccinium vitis-idaea) by use of thin-layer chromatography (TLC), image analysis, and UV–visible spectroscopy. The results were subjected to multivariate techniques, for example cluster analysis (CA), principal-components analysis (PCA), and linear discriminant analysis (LDA). Evaluation of the discriminating power of TLC coupled with digital image analysis for characterization of the supplements was performed by use of chemometrics. Zero-order, first-order, and normalized zero-order UV–visible spectra of extracts of fruit and dietary supplements were digitized and used in the chemometric analysis. To increase the relevance of the study, the dietary supplement extracts were compared with extracts of the natural fruit. Results obtained by application of CA, PCA, and PCA–LDA to the chromatograms suggest the possibility of discrimination among dietary supplements on the basis of the raw material used for their preparation.


20. Graziella L. Turdean, Dorina Casoni, Costel Sarbu, Structure–electrochemical properties correlations of some phenol derivatives investigated by electrochemical techniques, Journal of the Iranian Chemical Society, 2016, 13, 945-956. Abstract
A series of 25 phenol derivative compounds having potential antioxidant capacity were studied by cyclic voltammetry and square-wave voltammetry, in order to estimate the electrochemical parameters of the redox process occurring at the graphite electrode. A correlation between the structure of phenol derivatives, the peak potential, the HOMO–LUMO gap and their antioxidant activity lead to a qualitative classification of the studied compounds as a function of the increase of their antioxidant capacity.


21. Dorina Casoni, Costel Sarbu, Chromatographic approach for the evaluation of radical-scavenging activity using a new time-monitoring image analysis method, Journal of Planar Chromatography, 2016, 4, 299-305. Abstract
A thin-layer chromatographic (TLC) procedure combined with digital image processing analysis was developed for quantitative time-monitoring of the radical- scavenging activity. Reaction of 2,2-diphenyl-1-picrylhydrazyl (DPPH•) with reference antioxidants and selected catecholamine compounds was monitored on RP-18 chromatographic plates using dot-blot technique and different concentrations of standards. Images of the chromatographic plate were recorded after background staining every 5 min for 1 h using


Scientific papers related to the project topic, published in ISI journals


1. Sarbu, C., Nascu-Briciu, Rodica Domnica, Kot-Wasik, Agata, Gorinstein, Shela, Wasik, A., Namiesnik, J., Classification and fingerprinting of kiwi and pomelo fruits by multivariate analysis of chromatographic and spectroscopic data, Food Chemistry, 130 (2012) 994–1002. (FI = 3,334) Abstract
ABSTRACT


2. Tache, F., Nascu-Briciu, Rodica Domnica, Sarbu, C., Micale, Florina, Medvedovici, A.,Estimation of the lipophilic character of flavonoids from the retention behavior in reversed phase liquid chromatography on different stationary phases: A comparative study, Journal of Pharmaceutical and Biomedical Analysis, 57 (2012) 82-93. (FI = 2,947) Abstract
ABSTRACT


3. Cobzac, Simona Codruta, Casoni, Dorina , Fazakas, A. L., Sarbu, C., Determination of food synthetic dyes in powders for jelly desserts using slit-scanning densitometry and image analysis methods, Journal Liquid Chromatography & Related Technologies, 35 (2012) 1429-1443. (FI = 0,565) Abstract
ABSTRACT


4. Tuhutiu, Ioana, Casoni, Dorina, Sarbu, C., Comparative Study of Different TLC-Image Analysis Methods for Quantitative Evaluation of Parabens in Pharmaceutical Suspensions, Studia Universitatis Babes-Bolyai Chemia, 57 (2012) 83-93. (FI = 0,089) Abstract
ABSTRACT


5. Mot A.C., Coman Cristina, Miron Carmen, Damian, G., Sarbu, C., Silaghi-Dumitrescu, R., An assay for pro-oxidant reactivity based on phenoxyl radicals generated by laccase, Food Chemistry, 143 (2014) 214–222. (FI = 3,334) Abstract
ABSTRACT


6. Medvedovici, A., Albu, F., Nascu-Briciu, Rodica Domnica, Sarbu, C., Fuzzy clustering evaluation of the discrimination power of UV-Vis and (+/-) ESI-MS detection system in individual or coupled RPLC for characterization of Ginkgo Biloba standardized extracts, Talanta, 2014, 119, 524–532 Abstract
ABSTRACT

Papers in work