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Web of knowledge for NanoForOsteo Contract number EuroNanoMed2, no.4 - 005/2013 National Authority: UEFISCDI |
BABES BOLYAI UNIVERSITY OF CLUJ NAPOCA Multifunctional Injectable Nano HAp Composites for the Treatment of Osteoporotic Bone Fractures (NanoForOsteo) |
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· NanoForOsteo Project Results
NanoForOsteo Project: Multifunctional injectable nano HAp composites for the treatment of osteoporotic bone fractures
Results 2014:
Preparation of innovative nano materials, as said in WP1.1
and WP1.2, in a form suitable for the preparation of coated microcapsule for
drug delivery and for biological activity studies. The nano materials were
precipitated from water solution using advanced processing methods in order
to control crystallite size, morphology, and crystallinity of the final
product. Products were characterized via physical chemistry methods, X-ray
diffraction (XRD), transmission electron microscopy (TEM), atomic force
microscopy (AFM), FT-IR. These innovative nano materials, also named
NanoForOsteo materials, were sent and explored in WP2-WP6. The
multi-disciplinary joint research done within the NanoForOsteo project
represents a model of international excellence research and contributes to
the development of multifunctional injectable composites, with applicability
in the treatment of osteoporotic bone fractures.
The research was focused on the synthesis of innovative nano materials, multi substituted with various cations, which have to present the necessary properties to be applicable in the treatment of osteoporotic bone fractures. In order to achieve the objectives for 2015, the nano technologies developed in the previous WP1.1 and W1.2 from 2014 were optimized, with a particular emphasis on thermal treatments and various periods of time used for the synthesis of the designed innovative nano materials. The obtained NanoForOsteo materials were optimized both structurally and morphologically by advances technological processing, realized in the NanoForOsteo project, by activity WP1.3. The physical and chemical characterization of these optimized materials was achieved in WP1.3, by XRD, FTIR and Raman spectroscopy, TEM, AFM, SEM, and by BET analysis, in order to specify structure and morphology of the obtained nano powders. Within this stage, we also identified the role of nano materials on the behavior of cells, in activity WP1.4 in vitro. These innovative nano materials, also named NanoForOsteo materials, are biocompatible and were sent and explored in WP2-WP6.
Results 2016:
NanoForOsteo new materials were synthesized and physically
and chemically characterized and used as scaffolds in cell culture in total
agreement with WP1.4 and WP1.5. The optimum concentrations of essential
elements were determined within NanoForOsteo materials to have an enhanced
biological activity in cells culture of osteoblasts and consequently to be
appropriate for fracture treatment of osteoporotic bones. Also, the
osteoblasts performance on scaffolds realized from NanoForOsteo materials
were evaluated by collagen production, alkaline phosphatase activity,
production of osteopontin and osteocalcin, which are osteoblasts markers
essential for the development of new bones. Also, the actin fibers and
adhesion focal were visualized and the role of essential elements was
evaluated. Comparative studies were performed on NanoForOsteo powders and on
injectable multifunctional composites in cell cultures and the nano
materials with high performance were selected for the treatment of
osteoporotic bones. The obtained scientific and technological results were
presented and all deliverables were discussed in scientific demonstrative
sessions of consortium with participation of all partners in Babes-Bolyai
University of Cluj-Napoca (21 – 27 April 2016) and in University of Bordeaux
(17-22 November 2016). Periodically, the NanoForOsteo materials were
delivered as pastes and lyophilized powders, non-calcined or calcined, to
partner in Riga, Davos and in Bordeaux for further WP2- WP6 activities.
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